山桃稠李果实花色苷对Nrf2/Keap1通路的影响

刘荣; 姜元松; 辛越; 王蕾; 杨巍巍

doi:10.13386/j.issn1002-0306.2014.06.023

山桃稠李果实花色苷对Nrf2/Keap1通路的影响

Effect of Prunus Maackii fruit anthocyanins on Nrf2/Keap1 pathway

摘要

摘要: 目的:探讨山桃稠李果实花色苷对Nrf2/Keap1通路的影响。方法:质量浓度为0.05、0.2、0.8mg/mL的山桃稠李果实花色苷分别作用于HepG2细胞24、48、72h,MTT法检测对HepG2细胞生长抑制率,检测各组细胞内谷胱甘肽（GSH）含量及谷胱甘肽硫转移酶（GSH-ST）、磷酯酰肌醇-3-激酶（PI3K）活性变化;实时荧光定量RT-PCR实验方法检测山桃稠李果实花色苷对HepG2细胞的Nrf2、Keap1、GSTP1的基因表达的影响。结果:随着花色苷质量浓度的增加,HepG2细胞的谷胱甘肽（GSH）含量下降,谷胱甘肽硫转移酶（GSH-ST）、磷酯酰肌醇-3-激酶（PI3K）活性显著降低;Nrf2、Keap1、GSTP1的基因表达下调。结论:山桃稠李果实花色苷能够通过对Nrf2/Keap1通路的调节来降低HepG2细胞的抗氧化能力。

Abstract: Objective:To study the effect of Prunus maackii fruit anthocyanins on Nrf2/Keap1 pathway. Methods:The HepG2 cells were treatet with concentration of 0.05, 0.2, 0.8mg/mL Prunus maackii fruit anthocyanins solution for 24, 48, 72h respectively. The growth inhibition rate of HepG2 cells and the content of glutathione (GSH) , activity of phosph oinositol-3-kinase (PI3K) of each group cells were determined by the method of MTT. The real-time fluorescent quantitative RT-PCR method was used to study the effect of Prunus maackii fruit anthocyanins on Nrf2, Keap1, GSTP1 in HepG2 cells in genetic expression. Results:The results showed that as the content of anthocyanins increased, the content of GSH of HepG2 cells deceased, and the activity of glutathione s-transferases (GSH-ST) and PI3K decreased significantly. The Nrf2, Keap1, GSTP1 gene expression level dropped. Conclusion:the Prunus maackii fruit anthocyanins could reduce antioxidant capacity of HepG2cells by adjusting the Nrf2/Keap1 pathway.