响应面法优化野波罗蜜根皮总黄酮的微波提取工艺
Optimization of microwave extraction of flavonoids from wild Jackfruit root barks by response surface methodology
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摘要: 目的:运用响应面分析法优化野波罗蜜根皮总黄酮的微波提取工艺。方法:以野波罗蜜根皮为材料,采用微波提取法,提取野波罗蜜根皮总黄酮。在单因素实验的基础上,利用Box-Benhnken中心组合实验和响应面分析法,优选微波法提取野波罗蜜根皮总黄酮的工艺条件。结果:野波罗蜜根皮总黄酮的最佳提取工艺为:在60%的乙醇溶液中微波提取,液固比为52∶1mL/g,微波时间为2.2min;此条件下,野波罗蜜根皮总黄酮实际提取率为9.82%,预测值为9.80%。结论:运用响应面分析法优化得到的微波提取参数准确、可靠。Abstract: Objective:These optimum conditions for the microwave extraction of flavonoids from wild jackfruit root barks were determined using response surface methodology. Methods:Wild jackfruit root barks as the materials, flavonoids were extracted by microwave extraction method. On the basis of one-factor-at-a-time experiments, the process conditions of microwave extraction of flavonoids from wild jackfruit root barks were optimized by Box-Benhnken central composite test and response surface analysis method. Results:Results showed that these optimal extraction conditions were as follows:60% ethanol solution as extraction solvent, microwave extraction time of 2.2min, liquid-to-solid ratio of 52∶1mL/g. Under these conditions, the actual extraction rate of flavonoids from wild jackfruit root barks total was 9.82% in comparison with the predicted yield of 9.80%. Conclusion:The parameter of microwave extraction was optimized by response surface methodology are accurate and reliable.
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