Abstract: A pedicin-producin Pediococcus acidilactici strain PA003 was isolated from traditional fermented Chinese cabbage in our previous study. The healthy functions of PA003, such as antibacterial activity, high temperature tolerance, acid resistance, bile salt tolerance and medicine tolerance were tested in this study.The results showed that the strain was able to inhibit the growth of Listeria mononucleosis and so on.It was resistant to the temperature of 50℃, but the survival rate declined over 80℃.The number of bacteria reduced from 108CFU /mL to 106CFU /mL after 6h treatment in MRS culture of pH2.5, whereas it showed increasing trend if treated with the culture of pH3.5and pH4.5 for 4h.The strain was proved to have better tolerance to the bile salt concentration of 0.1% ⁰.4%, but grew worse if it was above 0.4%. The strain also indicated strong tolerance to antibiotics such as Norfloxacin, Gentamicin, Tetracycline, Penicillin and Rifampin.The results suggested that Pediococcus acidilactici PA003 might be used as a candidate probiotics in animal and even human in the future.