柱前衍生HPLC-UV-Q-TOF-MS分析黑灵芝多糖的酶解产物

The analysis of enzymatic products of Ganoderma atrum polysaccharides by pre-column derivatization HPLC-UV-Q-TOF/MS

摘要: 采用1-苯基-3-甲基-5-吡唑啉酮（PMP）对灵芝粗多糖酶解产物进行衍生,并利用液相-紫外检测器-高分辨四级杆串联飞行时间质谱法（HPLC-UV-Q-TOF-MS）检测分析灵芝多糖的单糖组成及其酶解产物。结果表明,灵芝多糖的单糖组成主要是葡萄糖、甘露糖和半乳糖、及少量的戊糖、岩藻糖和葡萄糖醛酸。经HPLC-Q-TOF-MS分析鉴定,β-葡聚糖酶的降解产物为单糖、二糖到五糖的低聚糖,证明灵芝多糖多存在β-糖苷键,而果胶复合酶将黑灵芝多糖主要降解成单糖。

Abstract: 1-phenyl-3- methyl-5- pyrazolone ( PMP) was applied to derivate polysaccharide degradation product.Then liquid chromatography with ultraviolet detector- high resolution quadrupole tandem time of flight mass spectrometry ( HPLC- UV- Q- TOF- MS) was used to detect and analyze monosaccharide compositions and enzymatic hydrolysate of Ganoderma atrum polysaccharide. The results showed that the monosaccharide compositions of Ganoderma atrum polysaccharides were mainly glucose, mannose, galactose. The degradation products of β-glucanase were monosaccharides and oligosaccharides from disaccharide to pentasaccharides, but Ganoderma atrum polysaccharides were mainly degraded into monosaccharides by pectic enzyme.