西洋参多糖的超滤分离及其免疫增强活性研究

杨修仕; 周闲容; 王丽君; 于晓娜; 石振兴; 任贵兴; 董川

doi:10.13386/j.issn1002-0306.2014.05.017

西洋参多糖的超滤分离及其免疫增强活性研究

Ultrafiltration separation and immune enhancing activity of American ginseng polysaccharide

摘要

摘要: 采用超滤法分离西洋参多糖并研究其免疫增强活性。采用不同分子截留量的超滤膜将西洋参根水提物分为>100ku（AGP1）、30¹00ku（AGP2）、10³0ku（AGP3）和<10ku（AGP4）四个多糖组分,测定了四个多糖组分的总糖含量、单糖组成及免疫增强活性。结果表明:西洋参多糖的得率为5.17%,其中,AGP1^AGP4分别约占46.61%、3.30%、12.11%和37.98%。四个多糖组分的总糖含量范围为42.87%⁵6.55%。单糖组成分析表明,各组分分别由6⁸种单糖组成,且均含有阿拉伯糖、鼠李糖、甘露糖、半乳糖、葡萄糖和葡萄糖醛酸。免疫增强活性研究表明,AGP1与AGP2可显著促进巨噬细胞NO、TNF-α、IL-6及IL-10的生成,具有较强的免疫增强活性。

Abstract: The objective of this study was to separate polysaccharides from American ginseng by ultrafiltration and evaluate their immune enhancing activity. Hot water extract of the root was separated by membrane ultrafiltration into four different molecular weight fractions as follows: AGP1 ( > 100ku) , AGP2 ( 30¹00ku) , AGP3 ( 10³0ku) and AGP4 ( < 10ku) . Total yield of American ginseng polysaccharides was 5.17%, with the percentage of 46.61%, 3.30%, 12.11%, and 37.98% for AGP1^AGP4, respectively.Total sugar content of them was ranged from 42.87% to56.55%. Monosaccharide analysis showed that AGP1 ~ AGP4 were composed of six to eight monosaccharides, which all contained arabinose, rhamnose, mannose, glucose, and glucose acid. AGP1 and AGP2 showed remarkable immune enhancing activity by stimulating macrophages to produce NO, TNF-α, IL-6 and IL-10.