Abstract: The effects of different types of macroporous resin on hydrolysis soybean peptide desalination and its optimal condition were studied.In this experiment, hydrolysis soybean peptide was the research object.5 different kinds of macroporous resin---DA201-C, AB-8, D3520, D4006 and NAX-Ⅱ were used for studying desalting effect of hydrolysis soybean peptide. The experiment showed that DA201- C had the highest adsorption- desorption capability. In further study, the optimal desalination conditions of soybean peptide with the DA201- C were confirmed as follows: pH of soybean peptide 4.5, concentration of hydrolysats 60mg /mL, ethanol elution concentration 75%, under these conditions, the desalination rate reached about 93.23%, the recovery of soybean peptides was about 88.5%.