乳糖酸产生菌的发酵培养基优化

Optimization research of fermentation mediums for Lactobionic acid producing strain

摘要: 以乳糖酸生产菌Rsoultella terrigena Y20为实验菌株,在单因素实验的基础上进行Plackett-Burman实验和BoxBehnken实验设计,采用响应面法建立土生拉乌尔菌Rsoultella terrigena Y20的发酵培养基优化模型。得到的最优产乳糖酸培养基为:乳糖110.28g/L,蛋白胨19.00g/L,硝酸铵3.00g/L,MgSO40.625g/L,K2HPO41.25g/L,KH2PO41.0g/L,NaCl 0.625g/L,pH7.5。经实验验证,在此条件下,乳糖酸的产量可达102.614g/L,转化率为93.05%,比优化前的93.84g/L提高了9.35%。

Abstract: Using Lactobionic Acid producing strain as test strain, on the basis of single factor experiment to conduct Plackett-Burman and Box Behnken experiment design, response surface method was adopted to establish the Rsoultella terrigena Y 20 fermentation medium optimization model. Optimal fermentation conditions for the highest Lactobionic Acid content in fermentation medium were set at lactose 110.28g /L, peptone 19.00g /L, NH 4 NO 3 3.00g /L, MgSO 4 0.625g /L, K 2 HPO 4 1.25g /L, KH 2 PO 4 1.0g /L, NaCl 0.625g /L, pH7.5. The results showed that under these conditions, Lactobionic Acid's yield was up to 102.614g /L, conversion ratio was 93.05%, which was 9.35% higher than that of non-optimized medium which was 93.84g /L.