原生质诱变选育高产酸性α-淀粉酶黑曲霉菌株

Production of acid α-Amylase Aspergillus Niger strains by mutagenesis of protoplast

摘要: 以产酸性α-淀粉酶的黑曲霉0-2-1为出发菌,选用180s的紫外线照射剂量对其孢子原生质体进行诱变,利用固体平板透明圈法初筛和发酵液酶活测定复筛;筛选到3株酶活有较大提高的突变株UV9、UV17、UV31,其酸性α-淀粉酶活力分别为184.66、162.73、167.31U/mL,比出发菌株分别提高了54.9%、38.1%和42.0%,遗传稳定性实验证明3株突变株传代过程中均保持相对稳定的较高酸性α-淀粉酶活力。

Abstract: Aspergillus Niger 0-2-1 was used as original strain.This strain of separation and purification of spores protoplast was treated by Ultra Violet ( UV) . The mutant with the bigger ratio of hydrolyzation circle diameter to bacterial colony diameter was screened so as to assay acid α- amylase activities. After genetic stability testing, three strains ( UV9, UV17, UV31) with an high activity of acidα-amylase were screened.Strain UV9, UV17 and UV31 were obtained with an acid α- amylase activity of 184.66, 162.73 and 167.31U /mL.The activities obtained were 54.9%, 38.1% and 42.0% higher than that of the parent strain.