Abstract: The phase soluble diagram of α-, β-, γ-cyclodextrin with natamycin was analyzed. Results showed thatα- and β- cyclodextrin gave AL type, and the inclusion constants were calculated as 3.803 ×10-5and 1.362×10-3, separately. While γ-cyclodextrin gave AN type. The water solubility enhancement folds for α-, β-, γ-cyclodextrin were 4.8, 15.5, 104 folds, respectively. Furthermore, composite cyclodextrin could also form inclusion with natamycin, and the water solubility enhancement was calculated as 157 folds. The inclusion rate of composite cyclodextrin for natamycin was 57.15%. And the retention rate was 98.8% for natamycin in composite cyclodextrin after storing at 60℃ for 6 days. This study will append applied scope in food for natamycin.