Abstract: The ultrasonic-microwave synergistic extraction method of α-glucosidase inhibitor from leaves of Salix babylonica. L was investigated. Based on single factor test, the orthogonal test was designed to optimize the extraction conditions. Results showed that the optimum process was as following:microwave power 150W, solid-liquid ratio 1 ∶18g/mL, extraction time 270s and alcohol concentration 70%. Under these conditions, the inhibition rate of α-glucosidase reached 77.85%. Compared with other extraction methods, the ultrasonic-microwave synergistic extraction had obvious advantages in the extracting effect. Compared with other materials, leaves of Salix babylonica. L showed stronger inhibition to α-glucosidase.