Abstract: The relationship among protease activity, soluble protein content and quality in antarctic krill (euphausia superba) was discussed under different storage temperature and storage life. TVB-N and total plate count went beyond the limits of spoilage after antarctic krill was stored under 10℃ for 15h, or stored under 4℃ for 36h. Even if the antarctic krill was stored under-20℃, it would also went spoilage with a rapid rate.-80℃ was the perfect storage temperature of the antarctic krill, which could keep its quality and activity under ultralow temperature. As the quality of antarctic krill declined, the TVB-N and total plate count went up logarithmically, the protease activity and soluble protein content decreased, moreover, they existed significant association during storage process under 10℃, 4℃ and-20℃. Protease activity, TVB-N and total plate count were not significantly correlated. Soluble protein, TVB-N and total plate count on the LOD scores existed significant correlation.