响应面法优化Bacillus amyloliquefaciens ZJHD-06产类细菌素发酵培养基
Optimization of fermentation medium of Bacillus amyloliquefaciens ZJHD-06 by response surface methodology
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摘要: 以分离自海洋宝石石斑鱼肠道的一种解淀粉芽孢杆菌ZJHD-06所产类细菌素为研究对象,以单核增生李斯特菌为抗菌活性测试指示菌,以抑菌圈直径大小为指标,采用响应面法对菌株ZJHD-06产类细菌素的发酵培养基成分进行优化。通过Plackett-Burman实验筛选出对类细菌素产量具有显著影响的因素,再通过最陡爬坡实验确定显著因素的中心水平,最后进行Box-Behnken实验设计,最终得到的最佳配比为葡萄糖浓度4.80%,蛋白胨浓度0.47%,酵母浸膏浓度0.44%,NaCl、K2HPO4和MgSO4·7H2O浓度分别为1.0%、0.01%、0.03%。在该培养基配比下,发酵上清液的抑菌圈直径增加了27.8%。Abstract: The fermentation medium of Bacillus amyloliquefacien ZJHD- 06 isolated from intestine of Epinephelus areolatus was optimized for a bacteriocin-like substance ( BLS) production.Bacteriostatic circle diameter was used as evaluation index against L.monocytogenes ATCC 35152.Firstly, the notable factors were screened by Plackett- Burman experiment design.Then, the path of steepest ascent was undertaken to approach the optimal region of the significant factors.Finally, Box-Behnken design was adopted to identify optimal proportion.The results showed the optimal proportions were glucose 4.80%, peptone 0.47%, yeast extract 0.44%, NaCl 1.0%, K 2 HPO 4 0.01% and MgSO 4 ·7H 2 O 0.03%. The bacteriostatic circle diameter of the fermentation supernatant was increased by 27.8% using the optimal proportions.
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