蚕蛹蛋白和蚕蛹短肽的模拟胃肠道消化特性研究
Nutritional characteristics of silkworm pupae protein and peptides after simulated gastric and simulated intestinal digestion in vitro
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摘要: 研究了蚕蛹蛋白和蚕蛹短肽的氨基酸组成,并分析了其在模拟胃肠道消化过程中的消化特性和氮释放量,探究蚕蛹蛋白和短肽在人类营养支持方面的应用。使用自动氨基酸分析仪测定蚕蛹蛋白和蚕蛹短肽的氨基酸含量,并计算氨基酸评分(AAS)、化学评分(CS)和必需氨基酸指数(EAAI)。蚕蛹蛋白和蚕蛹短肽的消化特性和氮释放量通过体外模拟胃肠道消化实验和凯氏定氮法进行评价的计算。结果表明,蚕蛹蛋白和蚕蛹短肽含有人体所需的18种氨基酸,而且必需氨基酸的组成合理。蚕蛹蛋白和蚕蛹短肽在体外模拟胃肠道消化实验中有良好高效的消化率和较高的氮释放量(约90%)。因此在今后将蚕蛹蛋白和蚕蛹短肽作为优质且经济的蛋白质原料,在动物饲料和人类肠内营养支持制剂方面有巨大的应用潜力。Abstract: This study was designed to evaluate the nutritional characteristics and percentage of released nitrogen ( %) of silkworm pupae proteins and peptides after simulated gastric and intestinal digestion, with a view to use in animal feeds or human nutritional support.Distribution of amino acids in the processed protein was analyzed and the amino acid score, chemical score, and essential amino acid index ( EAAI) were calculated. The method of simulated gastrointestinal digestion in vitro and Kjeldahl determination were used to analyze absorption characteristics and the released nitrogen ( %) of samples. Silkworm pupae proteins and peptides contained all amino acids required by humans, and the composition was reasonable. Simulated gastrointestinal digestion resulted in about 90% nitrogen release amount. The data indicated that this protein might be useful as an economic, high quality supplement in animal feeds and for human nutritional support.
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