Abstract: Trichloroacetic acid method, Sevag method and the protein isoelectric point method were used to remove protein from Opuntia dillenii Haw.crude polysaccharides.And deproteinization effects of this three methods were compared by the loss of protein, the loss of polysaccharides and the loss of inoxidizability.Results showed that with concentration of trichloroacetic acid 3%, deproteinization was most effective.In this condition, the loss of protein was 80.28%, the loss of polysaccharides was 5.98% and the loss of inoxidizability was 34.56%.