Abstract: Two strains of bacteria and one strain of fungus were isolated from decayed Enteromorpha proliferat.Based on the gene sequence the microorganisms were Bacillus cereus, Bacillus subtilis, and Saccharomyces cerevisiae, respectively.The complex microbial inoculants named as EM-D (including Bacillus cereus, Bacillus subtilis, Saccharomyces cerevisiae and one strain of Aspergillus oryzae) was applied on the experiment of Enteromorpha prolifera fermentation.The results showed the fermentation process possesses characteristics of warming rapidly, high temperature, short period, completely degradation of the tissue and indistinctive odor.The fermentation production meets the relevant standards of the national organic fertilizer and could be used as fertilizer directly.Green algae bio-organic fertilizer which was produced by the fermentation production and organic materials was rich in N, P, K.The fertilizer was also composed of active polysaccharides, amino acid and the microelement of I, Mg, Mn, Ti.Compared to the control group, the fertilizer amount of 4t/hm2could significantly increase the weight, diameter, reduce sugar content and chlorophyll content of Cherry Radish.At the same time, the application of green algae biological fertilizer could reduce of chemical fertilizer usage, improve soil microbial environment, and increase of soil organic component content.