Abstract: In this study, the adsorption and elution parameters was studied during extraction and separation of octopamine from fish sauce, while the static and dynamic adsorption was combined and adsorption and desorption rate were also considered.The results demonstrated that 60¹00 mesh silica gel had high efficiency in separate adsorption with the static saturated adsorption of 3.837mg·g- 1 (dry silica gel) .30% ethanol was as eluent with its elutive power 90.48%.The optimal value of the dynamic adsorption of octopamine in fish sauce on 60¹00 mesh silica gel was 4BV, and when the volume of eluent was 1.1BV, the eluting process was basically completed.The eluate was refined by a HPLC separation system.White solid powder was obtained after vacuum freeze drying.This white powder and octopamine standard was analyzed by mass spectrometer under the same condition.It can be proved that the white powder was natural octopamine with high purity.