大米蛋白抗氧化肽的活性以及组成鉴定研究

Enzymatic preparation of antioxidant peptides from rice protein and identification of antioxidant peptides

摘要: 采用碱性蛋白酶酶解大米蛋白制备大米蛋白抗氧化肽。研究表明大米蛋白酶解产物具有螯合金属铁离子、清除H2O2、羟自由基、DPPH自由基、抑制亚油酸自然氧化的作用。通过应用高解析离子淌度质谱（HD-MS）分析鉴定米蛋白抗氧化肽的序列主要为Met-Pro-Pro-Ser-Ser-Pro-His（376.68u）,Leu-Ala-Gly-Pro-Lys-Phe-Ala-Leu（408.75u）和Met-Pro-Arg-His-Asp-Pro-Gln（440.70u）。

Abstract: This antioxidant peptides prepared from rice protein hydrolyzed by Alcalase were characterized for their antioxidant properties in vitro reflected by the abilities to scavenge hydrogen peroxide as well as hydroxyl and DPPH free radicals, to chelate iron ions, and to inhibit autooxidation of linoleic acid.The antioxidant peptides were identified to be Met-Pro-Pro-Ser-Ser- Pro- His (376.68u) , Leu- Ala- Gly- Pro- Lys- Phe- Ala- Leu (408.75u) , and Met-Pro-Arg-His-Asp-Pro-Gln (440.70u) by High Definition Mass Spectrometry (HD-MS) .