HPLC法测定不同品种蘑菇中麦角硫因的含量

HPLC determination of ergothioneine in mushrooms of different species

摘要: 10种不同品种蘑菇中的麦角硫因经加热回流提取,过滤,使用氨基柱,以乙腈∶5mmol/L醋酸铵(80∶20)为流动相进行分离,用外标法峰面积定量。结果显示:麦角硫因在0.005~0.106mg/mL范围内峰面积与质量浓度的线性关系良好,高、中、低浓度(0.011、0.014、0.017mg/mL)的平均回收率分别为102.0%、100.2%、101.5%,10种蘑菇中麦角硫因的含量范围为0.000%~0.280%,所用方法简便、准确,可用于测定不同蘑菇品种中麦角硫因的含量,为该稀有氨基酸的应用奠定基础。

Abstract: Samples were extracted with water by heating reflux and the extract was filtration.The supernatant was then analyzed by NH 2 column with elution (acetomitrile- 5mmol /L ammonium acetate solution (80 ∶ 20) as mobile phase) and quantified by external standard method.A good linearity was shown in the range of 0.005⁰.106mg /mL.The average recoveries at three spike levels (0.011, 0.014, 0.017mg /mL) were 102.0%, 100.2% and 101.5%, respectively.The contents of ergothioneine in mushrooms at three different concentration were in the range of 0.000% ⁰.280%.The method was simple and reliable, and might be used for the determination of ergothioneine in the mushrooms of different species and the foundation for the application of rare amino acids.