Abstract: As a kind of green food catalyst, lipoxygenase exists widely in plants and microorganisms. The method of macroporous resin was chosen to separate lipoxygenase from its fermented product of the genetic engineering bacteria PET-32a-ana-LOX. The results showed that the HPD600 resin had a strong selective adsorption ability to lipoxygenase. When the sample amount of 4.3BV, the concentration of 4.026mg/mL, and adsorption flow rate of 1.0BV/h was adopted, its adsorption rate was 96.8%. When the compound eluent (ratio of ethanol to ethyl acetate, 1) , its amount of 33.3BV and rate of 4.0BV/h was operated, its elution rate, yield and specific activity was 94.8%, 95.0% and 6.2 times respectively, which could provide scientific references for the lipoxygenase production.