Abstract: Effects of temperatures, pH and metal ions on lipase activity in goat milk lipase were studied in this paper. The results showed that the lipase activity for goat milk was highest at 37℃ and lipase had higher thermal stability at 40 ⁵0℃. The optimal pH of lipase was 6.5, and stability was higher at pH5.0 ⁶.5. The lipase activity for goat milk was activated by Ca2+and Fe2+, inhibited by K+、Zn2+、Cu2+、Mn2+and Mg2+. The lipase activity was inhibited at lower concentrations of Al3 +and Fe3 +, however, it could be activated at higher concentrations of Al3+and Fe3+.