Abstract: A confirmative method was developed to detect 6 biogenic amines in traditional soybean products by HPLC-MS/MS. Biogenic amines were extracted with acetocaustin. The separation of biogenic amines were carried out based on Sunfire Atlantis HILIC column (150.0mm×2.1mm, 3μm) , with a gradient elution of acetonitrile and water (including 2mmoI/L ammonium acetate, 0.1% formic acid) . The detection method of electrospray ionization source with positive-ion electrospray ionization mode was used for mass spectrometric analysis.Qualitative and quantitative analysis of the 6 biogenic amines were completed within 15min. Experimental results showed that the biogenic amines had a good linearity (r>0.991) in the range of 0¹000μg/kg. The limit of detection (LOD) was 10⁵0μg/kg. The limits of quantification were 33.3¹66.6μg/kg. The average recoveries were 72.5%¹08.2%. The relative standard deviations were 6.2%¹3.2%. The results showed that it was a simple, rapid, accurate and highly sensitive method to analyze and detect the biogenic amines in the traditional soybean products.