冻藏对海湾扇贝闭壳肌蛋白质变性及组织结构的影响
Effects of frozen storage on protein denaturation and structure of Argopecten irradiams muscle
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摘要: 以海湾扇贝闭壳肌盐溶性蛋白含量、肌原纤维蛋白Ca2+-ATPase活性及组织的显微镜观察为指标,研究了冻藏条件对海湾扇贝闭壳肌蛋白质变性及组织结构的影响。结果表明,冷藏过程中蛋白质发生变性,盐溶性蛋白及肌原纤维蛋白Ca2+-ATPase活性显著下降（p<0.05）;贮藏1575d时间内,（-35±1）℃下的闭壳肌盐溶性蛋白含量高于（-25±1）℃,且有显著差异（p<0.05）;贮藏过程中,（-35±1）℃下闭壳肌的肌原纤维蛋白Ca2+-ATPase活性高于（-25±1）℃,除贮藏0d、45d差异不显著外,其余相同贮藏时间内不同冻藏温度对肌原纤维蛋白Ca2+-ATPase活性影响差异显著（p<0.05）。（-35±1）℃冻藏条件下海湾扇贝闭壳肌肌肉纤维相对（-25±1）℃冻藏组织结构更加完整。因此,选择（-35±1）℃冻藏海湾扇贝闭壳肌对蛋白质及组织结构的变化影响较好。Abstract: Changes of salt solubility protein, the Ca2+-ATPase activity in myofibrillar protein and the structure of Argopecten irradiams muscle were studied during storage at two different temperatures ( (-25±1) ℃, (-35±1) ℃) .Results showed that during frozen storage, salt solubility protein and the Ca2 +-ATPase activity in myofibrillar protein was reduction signifienatly (p<0.05) . Argopecten irradiams muscle storage at two different temperatures ( (-25±1) ℃, (-35±1) ℃) ) , salt solubility protein changed signifienatly during storage time at 1575d (p<0.05) .In the process of storage, the Ca2 +-ATPase activity in myofibrillar protein changed signifienatly at different frozen storage temperatures except storage time at 0d and 45d (p<0.05) . Structure of Argopecten irradiams muscle frozen storage temperatures at (- 35 ± 1) ℃ was more complete than the storage temperatures at (-25±1) ℃. So (-35±1) ℃ was better on protein denaturation and structure of Argopecten irradiams muscle.
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