南蛇藤中齐墩果酸和熊果酸的提取工艺优化及毛细管电泳检测其含量分布

张颖; 崔英杰; 郭守东; 司艳红; 秦树存

doi:10.13386/j.issn1002-0306.2013.22.018

南蛇藤中齐墩果酸和熊果酸的提取工艺优化及毛细管电泳检测其含量分布

Optimization of the extraction process followed by capillary zone electrophoresis for the simultaneous determination of oleanolic acid and ursolic acid in Celastrus orbiculatus Thunb.

摘要

摘要: 利用响应曲面法优化南蛇藤中齐墩果酸（OA）和熊果酸（UA）的超声提取工艺-联用,建立毛细管电泳技术测定两化合物在南蛇藤不同部位中的分布。结果显示,齐墩果酸和熊果酸的最佳提取条件为采用80%甲醇,料液比20mL·g-1,超声提取时间为50min。齐墩果酸在根、茎、皮、叶中的含量分别为0.2561、0.6748、0.5190、0.1832mg·g-1,熊果酸在根、茎、皮、叶中的含量分别为0.3720、0.1558、0.1097、0.0677mg·g-1。结果表明,南蛇藤因用药部位不同各化学成分存在显著差异,响应曲面优化物质提取工艺具有简便、高效、节约试剂等优点,毛细管电泳技术是一种线性关系好、精密度高、准确、快速测定物质含量的简便方法。该研究为进一步开发南蛇藤的用药资源提供了理论依据。

Abstract: An optimized response surface methodology ( RSM) method and an efficient capillary zone electrophoresis (CZE) analysis method were developed for fast extraction and simultaneous determination of oleanolic acid (OA) and ursolic acid (UA) in Celastrus orbiculatus Thunb. (C.orticulatus Thunb.) . The results displayed that the optimized conditions to extract and determine OA and UA simultaneously in C.orticulatus Thunb. were 80% methanol, solvent to material ratio 20mL·g-1and extraction time 50min.Under the optimized UAE method and an efficient CZE analysis method, the contents of OA in root, stem, bark and leaf of C.orticulatus Thunb. were 0.2561, 0.6748, 0.5190, 0.1832mg·g-1. The contents of UA in root, stem, bark and leaf were 0.3720, 0.1558, 0.1097, 0.0677mg·g-1. The results showed that there were significant variations in the contents of OA and UA in different parts of C.orbiculatus Thunb, RSM was a handy extraction method with higher yield and lower solvent consumption. The CZE analysis method was validated to have good linearity, precision, reproduction and accuracy. The study provided theoretical foundation for developing medicine resource of C.orticulatus Thunb..