龙须菜中多糖含量测定方法的比较研究

杨贤庆; 刘名求; 戚勃; 邓建朝; 郝淑贤; 岑剑伟; 周婉君

doi:10.13386/j.issn1002-0306.2013.22.017

龙须菜中多糖含量测定方法的比较研究

Comparison of methods in determination of polysaccharide in Gracilaria lemaneiformis

摘要

摘要: 采用热水浸提和乙醇沉淀的方法提取龙须菜多糖,然后分别运用蒽酮硫酸法和苯酚硫酸法测定多糖含量,比较两种测定方法得到数据之间的差异和准确性。结果表明,蒽酮硫酸法在20⁸0μg范围内线性关系良好（R2=0.9992）,苯酚硫酸法在10¹00μg范围内线性关系良好（R2=0.9993）;龙须菜多糖含量测定结果显示蒽酮硫酸法偏高于苯酚硫酸法,苯酚硫酸法结果稳定性、重现性较好,加样回收率为99.76%⁹9.97%,RSD为0.22%⁰.25%,此结果较优于蒽酮硫酸法。综上,蒽酮硫酸法和苯酚硫酸法均能测定龙须菜多糖含量,但相比较而言,苯酚硫酸法更为合理准确,在龙须菜多糖含量测定时可优先考虑苯酚-硫酸法。

Abstract: Polysaccharideof Gracilaria lemaneiformis was extracted with hot water and sedimentated with ethanol.And then the content of polysaccharides was determined by anthrone-sulphuric acid method and phenol-sulphuric acid method, in order to compare the difference of these two methods. The linearity of the phenolsulphuric acid method (R2=0.9992) was 20⁸0μg and anthrone-sulphuric acid method (R2=0.9993) was 10¹00μg. At the same time, the result of phenol-sulphuric acid method had good stability, reproducibility and sample recovery with 99. 76 % ~ 99. 97 % and RSD was 0. 22 % ~ 0. 25 %, which was better than that of the anthrone-sulphuric acid method. In conclusion, both the anthrone-sulphuric acid method and the phenol-sulphuric acid method could determine the polysaccharide content of sample. However, the result of phenolsulphuric acid method was more reliable and accurate, it could be considered as the optimum assay method for polysaccharides in Gracilaria lemaneiformis.