复合乳酸菌对肠出血性大肠杆菌O157∶H7抑制作用的研究

王菲; 张继伦; 宁喜斌

doi:10.13386/j.issn1002-0306.2013.21.047

复合乳酸菌对肠出血性大肠杆菌O157∶H7抑制作用的研究

Antibacterial effect of compound lactobacillus on E.coli O157∶H7

摘要

摘要: 本研究利用实验室保存的7株常见乳酸菌进行抑制大肠杆菌O157∶H7实验,其中4株乳酸菌对大肠杆菌O157∶H7有明显抑制效果,分别为嗜酸乳杆菌(SL1)、鼠李糖乳杆菌(SL2)、干酪乳杆菌(SL3)、植物乳杆菌(SL4)。将4株乳酸菌混合后不但抑菌效果强于4株任意单独菌株,其发酵滤液pH相比4株单独菌株下降速度最快,且pH最低。说明混合后的乳酸菌产酸能力也明显增强。将SL1、SL2、SL3、SL4通过排列组合(C(n,m))可分成11组混合菌液,然后通过牛津杯抑菌方法进行抑菌实验,结果显示乳酸菌组合(SL2、SL3、SL4)具有最佳抑菌效果。再将SL2、SL3、SL4按正交设计方案以不同比例的接种量混合成复合菌剂进行抑菌实验,结果表明:乳酸菌组合(SL2、SL3、SL4)以3∶1∶3比例制备复合菌剂时,对大肠杆菌O157∶H7的抑制效果最好。

Abstract: Used the 7 lactic acid bacteria strains preserved in Lab to carry on the inhibited E. coli O157 ∶ H7 test separate. There were four lactobacillus: Lactobacillus acidophilus ( SL1) , Lactobacillus rhamnosus ( SL2) , Lactobacillus casei ( SL3) , Lactobacillus plantarum ( SL4) showed apparent antagonistic effect against E. coli O157∶H7.After mixing the four lactobacillus not only had the better inhibitory effect, the pH showed the fast decline, with the final value significantly lower than those of other cultures.The 11 composes from ( SL1, SL2, SL3, SL4) were design to inhibit E.coli O157∶H7 by oxford cup.Then the best combination were inhibited E.coli O157∶H7 in different proportions by orthogonal desigh.The results showed that: lactobacillus combination ( SL2, SL3, SL4) by 3∶1∶3 had the best antagonistic effect against E.coli O157∶H7.