梭柄松孢菇子实体中多糖组分及体外抗氧化活性分析

Analysis of polysaccharide composition and in vitro antioxidant activities of fruiting bodies of Catathelasma ventricosum

摘要: 采用热水浸提、85%、95%乙醇分级醇沉制备了两种梭柄松孢菇子实体多糖成分:CVP-85和CVP-95,分析了单糖组成,并测定多糖的体外抗氧化能力。结果表明:CVP-85和CVP-95的得率分别为9.57%±0.23%和5.02%±0.12%;CVP-85中的主要单糖物质为甘露糖、葡萄糖、半乳糖、岩藻糖、葡萄糖醛酸、半乳糖醛酸;CVP-95中的单糖物质有甘露糖、鼠李糖、葡萄糖、半乳糖醛酸。多糖提取物表现出显著的还原能力、DPPH自由基清除能力、羟自由基清除能力、ABTS·清除能力,且在一定浓度范围内,随浓度的增加而显著增强。

Abstract: Hot water, 85%, 95% ethanol precipitation were used to extract polysaccharides from Catathelasma ventricosum ( CVP- 85 and CVP- 95) , the monosaccharide composition were investigated, and the in vitro antioxidant activities were determined.The results showed that the yield of CVP- 85 and CVP- 95 was 9.57% ±0.23% and 5.02% ± 0.12%, respectively; the CVP- 85 was composed of mannose, glucose, galactose, fucose, glucuronic acid and galacturonic acid, in contrast, CVP- 95 was composed of mannose, rhamnose, glucose and galacturonic acid.The polysaccharide extracts possessed significant reducing power, as well as the strong DPPH radical, hydroxyl radical and ABTS radical scavenging activity, and the antioxidant activities of the extracts were significantly positive correlated with polysaccharide content ( p < 0.01) .