大豆与大豆芽中异黄酮的含量、组成及分布比较研究
Comparison of the isoflavone content and isoflavone profile in soybean and germinated soybean
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摘要: 首先通过光度分析法对大豆发芽过程中异黄酮总含量的变化、大豆中的异黄酮在子叶和芽茎中的分布进行了研究,结果表明,大豆中异黄酮总含量在芽长为3cm时达到最高。豆芽芽茎中异黄酮的总含量显著高于大豆原料和豆芽子叶。液质联用结合串联质谱的分析表明,在豆芽子叶中染料木苷和丙二酰基染料木苷为主要的组分,两者之和占豆芽子叶中大豆异黄酮总含量的63%左右;芽茎中大豆苷和丙二酰基大豆苷为主要组分,两者之和占豆芽芽茎中大豆异黄酮总含量的59%以上。豆芽芽茎中苷元的含量也明显高于豆芽子叶和大豆原料,其中大豆素含量最高,占芽茎大豆异黄酮总含量的6.71%。该研究结果表明豆芽芽茎中的大豆异黄酮具有更高的生物可利用度,比较大豆原料可能是一种更好的异黄酮食物来源。Abstract: The change of total isoflavones content ( TIFC) during the germination of soybean had been studied through the photometric method.The results showed that the total isoflavones content in soybean increased after the germination.The germinated soybean with the bud length reached 3cm had highest TIFC.The TIFC in sprout of germinated soybean was much higher than that in raw seed and cotyledon.The isoflavone profile in cotyledon of germinated soybean was similar as in raw seed. Genistin class species was the major isoflavone in cotyledon of germinated soybean, which accounted 63% of the total amount of isoflavones.The dominated components in sprout were daidzin and malonyldaidzin, sum of them account for more than 59% of total isoflavones content.Compared to raw seed and cotyledon, higer aglycon contents in sprout was observed, among them daidzein had the highest content that accounted for 6.71% of total isoflavones content.
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