壳寡糖铁配合物的合成及抗氧化作用

郭芳宁; 李春超; 金黎明; 付宇婷; 胡文忠; 田密霞

doi:10.13386/j.issn1002-0306.2013.20.045

壳寡糖铁配合物的合成及抗氧化作用

Synthesis and antioxidant activity of chitooligosaccharide-Fe complex

摘要

摘要: 以三氯化铁和壳寡糖为原料,合成了壳寡糖铁配合物,产率为55.56%。原子吸收光谱法测定其中铁含量为27.60mg/g。利用紫外-可见光谱、红外光谱两种表征手段,证实了壳寡糖铁配合物的合成。采用邻苯三酚自氧化法、DPPH法测定壳寡糖铁配合物的抗氧化能力。结果表明,在实验设置的浓度范围内,壳寡糖铁配合物的抗氧化能力随着浓度的增加而增加,且壳寡糖铁配合物的抗氧化能力高于壳寡糖。2.0mg/mL的壳寡糖铁配合物对超氧阴离子自由基的清除率为59.84%,对DPPH自由基的清除率为65.76%。

Abstract: Ferric trichloride and chitooligosaccharide were used to synthesize chitooligosaccharide-Fe complex in this experiment by chemical method. The yield was 55.56%. The content of Fe was 27.60mg/g measured by atomic absorption spectrometry method. Chitooligosaccharide- Fe complex was characterized by visible ultraviolet (UV-VIS) and infrared (IR) techniques. The antioxidant activities in vitro of chitooligosaccharide-Fe complex were investigated by pyrogallol autoxidation and DPPH method. The results showed that in the setting concentration range, the antioxidant activities increased with the elevation of concentration. The antioxidant activities of chitooligosaccharide-Fe complex were better than that of chitooligosaccharide. The clearance rates of superoxide anion free radical and DPPH of 2.0mg/mL chitooligosaccharide-Fe complex were 59.84% and 65.76%.