混菌发酵甘蔗糖蜜产维生素B12和维生素B2的工艺优化
Optimization of the fermentation of Vitamin B12 and Vitamin B2 from cane molasses by mixture-culture fermentation
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摘要: 采用响应面分析法（RSM）优化谢氏丙酸杆菌和枯草芽孢杆菌混合发酵产维生素B12（VB12）和维生素B2（VB2）的工艺条件。在培养时间、糖蜜浓度、温度、接种量、装液量、发酵初始pH的单因素实验基础上,采用Box-Behnken实验设计,通过响应面分析,对混菌的发酵工艺条件进行优化。得出最佳工艺条件为:糖蜜浓度20%（v/v）,装液量100mL/300mL三角瓶,混菌接种量18%（v/v,单菌接种量1∶1）,发酵温度30℃,发酵时间72h,初始pH7.0,在此条件下VB12与VB2的产量分别为262.54mg/L和10.14g/L,产量较单菌培养时的最高产量分别提高了13.16%和12.42%。Abstract: Response surface methodology was used to optimize fermentation conditions for Vitamin B12 and Vitamin B2 from cane molasses by mixture-culture. Based on results of single factor experiments, Box-Behnken experiment design was used to analyze and optimize fermentation time, molasses concentration, temperature, vaccination quantity, installs liquid volume and initial pH value. Optimal fermentation conditions were determined as follows:molasses concentration 20% (v/v) , installs liquid volume 100mL/300mL flask, vaccination quantity18% (v/v) , temperature 30℃, fermentation time 72h, initial pH value 7.0. Further validation experiments showed that the production of Vitamin B12 and Vitamin B2 were 262.54mg/L and 10.14g/L, which were higher than single bacterium cultivation of maximum yield increased by 13.16% and 12.42% respectively.
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