多壁碳纳米管基质固相分散萃取-HPLC法测定蔬菜中的啶虫脒残留

Multi-walled carbon nanotubes and matrix solid phase dispersion extraction-HPLC for the determination of acetamiprid residue in vegetables

摘要: 建立了以多壁碳纳米管（MWCNTs）为萃取剂的基质固相萃取（MSPD）、HPLC测定蔬菜中啶虫脒残留的方法。将样品与工业级碳纳米管、无水硫酸镁混合研磨后装柱,并用乙酸乙酯洗脱,高效液相色谱法测定。采用C8柱（3.0mm×100mm,1.8μm）分离,甲醇-水（3∶2,v/v）为流动相,流速0.10mL/min,紫外检测波长250nm,进样量2.5μL,柱温为30℃。结果表明,该方法对蔬菜样品中啶虫脒的回收率为84.0%¹06.0%,精密度为0.1%¹2.5%,方法最低检出限为0.0085mg/L。该法简便、快速、准确。

Abstract: With multi-walled carbon nanotubes (MWCNTs) as extractant, a method based on MSPD, HPLC had been developed for the determination of acetamiprid residue in vegetables.Samples were homogenized with industrial-grade carbon nanotubes and anhydrous magnesium sulfate in a mortar, packed into a cartridge, eluted by ethylacetate, and analyzed by HPLC.The acetamiprid residue was separated on a C 8 (3.0mm ×100mm, 1.8μm) column, with methanol-water (3∶2, v /v) as the mobile phase at the flow rate of 0.10 mL /min, then detected by UV detector at wavelength 210nm.The injection volume was 2.5μL, and the column temperature was 30℃.The results showed that vegetable samples recoveries were between 84.0% ~ 106.0%, with the relative standard deviations (RSDs) of 0.1%¹2.5%.And the detection lmit was 0.0085 mg /L.The method was simple, rapid, and accurate.