Abstract: Objective:To study regeneration technology of CT-101 macroporous adsorption resin (CT-MAR) .Methods:Using the orthogonal test, the saturated adsorption and desorption rate of CT-MAR for pigment in the second mother liquor of threonine were taken as evaluation parameters to investigate the effects of renewable liquid concentration, soaking time, and volume ratio of alkali and alcohol on regeneration of CT-MAR through static experiment, and to study the influence of flux, solvent amount and temperature on regeneration of CT-MAR through dynamic experiment.Alkaling ethanol was regenerant.Results:The optimal static technology was that the concentrations of NaOH and ethanol were 4% and 65%, respectively, the soaking time was 4h and the volume ratio of alkali and alcohol was 3 ∶7.The optimal dynamic technology was that the flow rate and volume of renewable liquid were 1.5BV/h and 3.5BV, respectively, and the operating temperature was 30℃.Conclusion:CT-101 macroporous adsorption resin is easy to regenerate.