戊糖乳杆菌的鉴定及其发酵D-乳酸的研究

Identification of Lactobacillus pentosus and its D-lactic acid fermentation

摘要: 分离得到1株乳酸杆菌LCA,根据其形态、生理生化性质和16S rDNA分子生物技术鉴定,被鉴定为戊糖乳杆菌（Lactobacillus pentosus）,其发酵产物经手性柱高效液相色谱法测定,含有光学纯度为80%以上的D-乳酸,并能利用D-木糖产生D-乳酸。筛选合适的D-乳酸生产菌株和研究D-乳酸的发酵技术对拓宽D-乳酸的应用范围具有重要意义。

Abstract: The LCA strain was identified as Lactobacillus pentosus according to the morphology, physiological characteristic and 16S rDNA.The fermentation products of LCA strain were analyzed by means of the chiral column HPLC.The optical purity of D-lactic acid was more than 80%.It was critical important for widening the range of application of D-lactic acid to screen suitable strain for D-lactic acid production and investigated technology of fermentation.