木瓜蛋白酶酶解南极磷虾壳提取虾青素的研究

Study on application of papain in astaxanthin extraction from antarctic krill shell

摘要: 以南极磷虾鲜虾壳类物质为材料,利用木瓜蛋白酶酶解提取虾青素,通过优化实验确定酶解的最优条件。本实验通过优化确定丙酮的最优浸提条件为料液比1∶32(g∶mL)、浸提温度39℃、浸提时间416min。通过优化实验确定木瓜蛋白酶酶解的最优条件为固液比1∶113(g∶mL)、酶底比6∶100、酶解温度50℃、酶解时间185min、pH=5。浸提酶解后的鲜虾壳类物质,每克干鲜虾壳类物质可提取总类胡萝卜素(虾青素)(350.709±12.060)μg,与丙酮直接浸提的(228.902±6.761)μg/g相比提高了53.214%。

Abstract: To explore a astaxanthin extraction process from hydrolyzing the antarctic krill shell by papain. Results through the optimization test the optimal acetone extraction conditions were sample/solution 1 ∶32 (g ∶mL ) , extraction temperature 39℃ , extraction time 416min. And the optimal hydrolyzing conditions of papain were solid∶liquid 1∶113 (g∶mL) , E/S 6∶100, temperature 50℃ , hydrolyzed time 185min, pH=5 by designing another optimization test. Under the experimental conditions , the amount of the total carotenoids (astaxanthin ) was (350.709±12.060) μg in per gram dried krill shell. The amount was higher 53.214% than the amount of acetone direct extraction (228.902±6.761) μg/g.