诃子粗提物及不同极性部位抑制低密度脂蛋白氧化修饰的研究

doi:10.13386/j.issn1002-0306.2013.16.025

诃子粗提物及不同极性部位抑制低密度脂蛋白氧化修饰的研究

Anti-low density lipoprotein oxidation of the crude extract and different fractions of Terminalia chebuLa Retz

摘要

摘要: 为了研究诃子抑制低密度脂蛋（Low density lipoprotein,LDL）氧化修饰活性,采用Cu2+体外诱导LDL氧化,以硫代巴比妥酸反应物（thiobarbituric acid reactive substance,TBARS）含量为指标评价氧化修饰程度。运用生物活性追踪法确定诃子抗LDL氧化修饰有效部位,并对其抗LDL氧化修饰活性进行了研究。结果表明,不同浓度诃子粗提物（0.5、0.9、2.7mg/mL）抗LDL修饰抑制率分别为64.31%、77.22%和82.66%。相同浓度（0.5mg/mL）的不同极性部位中,乙酸乙酯相抗LDL氧化修饰能力显著高于其他3个极性部位（p<0.05）,为有效部位。不同浓度有效部位（0.5、0.9、2.7mg/mL）抗LDL修饰的抑制率分别达73.19%、81.85%和86.29%。有效部位能明显延长LDL氧化反应潜伏期并能延缓和降低LDL氧化修饰程度。诃子抗LDL氧化修饰与其所含的总黄酮和总多酚有关。结果显示,诃子具有很强的抗LDL氧化修饰能力,为后期开发抗LDL氧化及抗动脉粥样硬化功能食品奠定基础。

Abstract: The property of anti-low density lipoprotein oxidation of Terminalia chebuLa Retz(TCR) was studied in vitro Cu 2 + induction on LDL. The effective fraction of TCR on the anti -LDL oxidation was determined by thiobarbituric acid reactive substance(TBARS) guided method. All these results were showed as follows:The inhibition rates of the TCR crude extracts on LDL oxidation were 64.31%,77.22% and 82.66%,respectively. The ethyl acetate fraction possessed the most strong inhibition activity on LDL than other three fractions(p<0.05), and was the effective fraction with the same concentrations. The inhibition rates of the effective fraction(0.5,0.9 and 2.7mg/mL) were 73.19% ,81.85% and 86.29% ,respectively. The incubation periods of oxidation were delayed and degrees of oxidation were reduced by the effective fraction with different concentrations. The property of anti-low density lipoprotein oxidation of TCR was related to the total flavonoids and polyphenols in it. This study showed that TCR possessed strong anti-LDL oxidation activity and lay the foundation for further exploration on anti-atherosclerotic functional foods.