模拟移动床色谱分离海藻糖和葡萄糖

Separating trehalose and glucose with simulated moving bed chromatography

摘要: 研究利用模拟移动床色谱分离海藻糖和葡萄糖的工艺过程,通过单柱实验测得相关参数,在线性条件下,应用三角形理论找到完全分离区,确定SMB的初始操作参数。通过实际操作优化,最终分离得到较高纯度的海藻糖(97.6%)和葡萄糖(98.8%),SMB的最佳工艺参数为切换时间11min、进料流量13.8mL/min、洗脱液流量29.7mL/min、萃取液流量20mL/min、萃余液流量23.5mL/min、循环流量17.6mL/min,系统操作温度为58℃。

Abstract: The separation processing of trehalose and glucose by simulated moving bed chromatography was studied.Related parameters were measured in single column experiment firstly.And the complete separation zone was found with triangle theory under linear condition.Higher purity of the trehalose (97.6%) and glucose (98.8%) were obtained through optimizing in SMB actual operation.Optimal SMB parameters were switching time 11min, feeding rate 13.8mL/min, desorbent solution flow rate 29.7mL/min, extraction solution flow rate 20mL/min, raffinate flow rate 23.5mL/min, and cycling flow rate 17.6mL/min.System operating temperature was 58℃.