扫频超声处理对玉米醇溶蛋白酶解特性的影响

毛舒云; 马海乐; 任晓峰; 骆琳

doi:10.13386/j.issn1002-0306.2013.14.054

扫频超声处理对玉米醇溶蛋白酶解特性的影响

Effect of pretreatment with sweeping frequency ultrasound on zymohydrolysis characteristic of zein

摘要

摘要: 为了探索扫频超声技术促进蛋白质酶解反应的效果,提高酶解产物的血管紧张素转换酶(ACE)相对抑制活性,利用扫频超声处理玉米醇溶蛋白,进行单因素考察来寻找最佳扫频超声工作模式、超声预处理参数和最佳酶解条件。在最佳的超声预处理及最佳的酶解条件下,ACE抑制率为48.48%、水解度为11.20%,酶解产物的IC50值为3.77mg/mL,比常规酶解(无超声处理)降低了31.20%,产品得率为66.15%,比常规酶解提高了14.39%。这表明扫频超声预处理能够有效地促进玉米醇溶蛋白的酶解反应,提高产物的ACE相对抑制活性。

Abstract: In order to explore the effect of sweeping frequency ultrasound (SFU) promoting zymohydrolysis reaction, improve the relative inhibitory activity (RIA) of hydrolysate to Angiotensin-I-Converting Enzyme (ACE) , the SFU was used to treat zein.The One-factorial experiments were taked to search the best operating mode of SFU, pretreatment parameters of ultrasound and the optimal conditions of hydrolysis.With the optimal conditions of ultrasonic pretreatment and hydrolysis, we could find out that ACE inhibitory activity was 48.48%, DH was 11.20% .The IC 50 value of hydrolysate was 3.77mg/mL, it decreased by 31.20%, compared with traditional enzymatic hydrolysis (without ultrasound treatment) .The yield of product was 66.15%, compared with traditional enzymatic hydrolysis, it improved by 14.39% .These results proved that SFU could effectively promoted enzymatic hydrolysis of zein, and improved RIA of hydrolysate to ACE.