竹荪多糖提取方法的比较研究
Comparative study on extraction methods of Dictyophora indusiata polysaccharide
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摘要: 以竹荪为原料,采用正交实验对超声复合酶法提取竹荪多糖工艺进行优化,并与热水提取法、超声波法、纤维素酶法、果胶酶法、木瓜蛋白酶法、复合酶法进行比较,结果表明,超声复合酶法提取竹荪多糖的最佳条件是料液比1∶50,酶解时间60min,酶解pH6,超声时间40min,多糖提取率为16.35%,而热水提取法多糖提取率为9.77%,超声波法为6.64%,纤维素酶法为8.84%,果胶酶法为10.06%,木瓜蛋白酶法为10.35%,复合酶法为11.27%,均低于超声复合酶法。故超声复合酶法提取竹荪多糖的提取率最高,所需时间较热水提取法大为减少,是7种方法中最好的提取方法。Abstract: The polysaccharide from Dictyophora indusiata was extracted by ultrasonic combined with multienzymatic method and the process was opitimized using orthogonal experimental design, compared with hot water method, ultrasonic wave method, cellulase method, pectinase method, papain method and multienzymatic method.The results showed that the optimal conditions of ultrasonic combined with multi-enzymatic extraction were material-water ratio 1∶50, enzymolysis time 60min, enzymolysis pH6, ultrasonic time 40min.The extraction ratio of Dictyophora indusiata polysaccharide was 16.35%, but it was 9.77% in hot water process, 6.64% in ultrasonic process, 8.84% in cellulase process, 10.06% in pectinase process, 10.35% in papain process and 11.27% in multi-enzymatic process, respectively, which were lower than that of ultrasonic combined with multi-enzymatic extraction.Therefore the extraction ratio of Dictyophora indusiata polysaccharide using ultrasonic combined with multi-enzymatic method was the highest, and it’s time of extraction was significantly shorter than that of hot water method.The ultrasonic combined with multi-enzymatic extraction was the best process among the 7 methods.
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