Abstract: To study the antioxidation activities of the different extract from cortinarius rufo-olivaceus via H22-bearing mice, discussed the possible mechanism of anti-tumor.The colorimetric analysis was adopted to determine the level of superoxide dismutase (SOD) , malondialdehyde (MDA) , calalase (CAT) and glutathione peroxidase (GSH-Px) in liver of H22-bearing mice after by giving petroleum ether, ethyl acetate, butanol phase extractions.Different extractions from corinairus rufo-olivaceus could increase the activities of SOD, MDA, CAT, GSH-Px and decrease the level of MDA especially the ethyl acetate part and polysaccharide.The result showed that the extract of corinairus rufo-olivaceus could obviously enhance the antioxidant acticity and prevent lipid peroxidation damage in H22-bearing mouse.