Abstract: Aspergillus species, especially Aspergillus niger and Aspergillus oryzae are widely used as expressed strains to produce a large number of protein products industrially.To increase the industrial production and achieve more economic benefits, it still needs to take measures to improve protein expression.There are many factors that may affect protein production, among which promoter efficiency is the most critical one.As strong promoters could be used to increase both homologous and heterologous protein expression at the transcriptional level, the choice and improvement of promoters in Aspergillus are very important. This review summarized some commonly used promoters in Aspergillus, and had focused on varied strategies to improve promoter efficiency. In addition, the application perspectives of modified promoters in protein expression had also been briefly described.