Abstract: The sustained-release antimicrobial packaging is a kind of material that has the special property of releasing active antibacterial component slowly.In this study, antibacterial complex was firstly prepared by forming inculsions between allyl isothiocyanate (AITC) and β-CD.And then β-CD-AITC complex, was mixed together with PLA and PEG.These blenders were then extruded and blown for making the antimicrobial packaging.The release of AITC and its antibacterial effect were mainly investigated. The obtained data suggested that, firstly, the heat-resistant ability of volatile antimicrobials had been improved markedly, as can be seen from the less loss rate of AITC about 75.56% when processing in extruder, compared with that of 98.77% before forming inclusions. Secondly, the release rate of AITC increased with the environmental temperature and humidity rising. Thirdly, the prepared antimicrobial packaging showed quite strong inhibitory effect on the main microorganism in food, especially on yeast and mould.