Abstract: Food grade glycerol monocaprylate studied microemulsion on common food-borne bacteria Staphyloccocus aureus, Escherichia coli antibacterial properties. The experiment showed that the minimum inhibitory concentration, MIC for Staphylococcus aureus and Escherichia coli food grade glycerol monocaprylate microemulsion were 135mL/L, had good antibacterial properties.Through the comparison of microemulsion and its components of the minimum inhibitory concentration, the inhibitory rate of antimicrobial activity antimicrobial properties of microemulsion, found mainly dependent on the antibacterial component glycerol monocaprylate, antibacterial and embedding effect of the micro emulsion but decreased glycerol monocaprylate bacteriostatic effect. However, as a kind of appearance of clear and transparent, thermodynamically stable food-grade antimicrobial delivery systems, food grade glycerol monocaprylate microemulsion has good market application prospect.