Abstract: The grape seed protein extracts were ultrafiltrated by the inorganic ceramic membrane tubes whose apertures were 100nm to concentrate the grape seed protein.The optimal condition of pressure of membrane, pH, ratios of liquid and material three factors were studied by orthogonal design.The results showed that the optimal conditions were the pressure of membrane at 0.1MPa, pH8, ratios of liquid and material at 1 ∶ 120. Under this conditions, protein recycle rate, concentration efficiency, and rate of pollution, membrane flux were 89.45% , 331.07L/m2·h, 7.26%, 350L/m2·h.Using different chemical reagent clean the membranes which had been contaminated, experiment testify that cleaning effect was optimization when using 0.75% HNO3 . Membrane flux recovery rate can be reached to 46.34%.