Abstract: Objective: The ultrasonic extraction technique of total flavones was optimized in order to provide a reference for extracting total flavones from Scutellaria baicalensis Georgi. Methods: On the basis of single factor experiments, the orthogonal experiment was used in the optimization of ultrasonic extraction technological parameters for obtaining good yield rate of total flavones.Results: The optimum conditions were as follows: the ratio of liquid to solid 14 ∶ 1 ( mL/g ) , emperature 60℃, methanol concentration 80% , ultrasonic time 50min. Under this condition, the yield of total flavones was 7.06%.And the effect of the four factors on the yield rate was as follows: liquid-to-solid ratio > extraction temperature > extraction time > solvent concentration. Conclusion: This ultrasonic extraction technique was simple and rapid, which had good yield rate.Furthermore, this study offered not only an accurate and effective reference standard for industrial applications, but also a good reference value for widely exploitation and utilization of Scutellaria baicalensis resource.