肉制品中沙门氏菌invA基因实时荧光定量PCR检测方法的建立

Establishment of real-time fluorescent PCR detection method of invA gene in salmonella

摘要: 针对沙门氏菌invA基因设计一对特异引物,建立SYBR Green实时荧光定量PCR检测方法,并进行特异性、敏感性、重复性检测。结果表明,所建立的沙门氏菌实时荧光定量PCR检测方法,特异性良好,组间组内重复性良好,沙门氏菌检测下线为101CFU/mL。本研究建立了沙门氏菌特异、敏感、快速的实时荧光定量PCR检测方法,为沙门氏菌的快速诊断奠定了基础。

Abstract: A pair of specific primers was designed according to invA gene of salmonella. The SYBR Green real-time PCR method for detecting salmonella was established. The specificity, sensitivity and repeatability of this method was analyzed. The result showed that this method were good specificity and repeatability, the detection of salmonella minimum copy number was 1×10CFU/mL. Therefore, the study established the salmonella specific, sensitive and rapid real-time fluorescence PCR detection method.