Abstract: An excellent esterifying function strain LZLJ 2103 was isolated and bred from ‘1573 National Treasures cellars’, which was identified by combining the morphological and molecular characteristics. After incubation for 7d, the white colony of LZLJ 21-3 became pink gradually as time went on. Colony surface clustered around curly mycelia. In the top of mycelium was the cleistothecium. The study of enzymatic properties of esterase from the strain showed that the optimal temperature for the enzyme was 40℃ and optimal pH was 5. 0. Na+, K+, Mg2+, Fe2+enhanced the enzyme activity, whereas Ca2+, Cu2+ inhibited the enzyme activity significantly. Internal transcribed spacers were amplified and sequenced. The 579bp sequence was compared with the known sequences in the GenBank database. Sequence alignment analysis has been done by bioinformatics software DNAMAN, DNAstar and Mega. The similarity of ITS between LZLJ 2103 and typical strain of Monascus purpureus was 99. 5% and the divergence 0. 5%. The phylogenetic analysis by NJ test showed that LZLJ 2103 came together with the known strains, which showed the highest similarity. According to morphological features and molecular analysis could draw the conclusion that LZLJ 2103 was belonged to one new strain of the Monascus purpureus.