粉末活性炭脱色豆粕蛋白酶解液的条件优化

Optimization of conditions for soybean meal protease hydrolysate decolourization with powdered activated carbon

摘要: 采用单因素和正交实验的方法,用粉末活性炭对豆粕蛋白酶解液进行脱色处理,以脱色率和肽保留率为指标,考察pH、粉末活性炭用量、脱色温度和吸附时间等因素对脱色结果的影响。结果表明:40mL豆粕蛋白酶解液在pH4.0、粉末活性炭用量0.6%、脱色温度50℃、吸附时间50min的条件下脱色效果明显,但有一定的肽损失。在此条件下脱色率为86.52%,肽保留率为75.04%。

Abstract: Powdered activated carbon was applied to decolour soybean meal protease hydrolysate by single factor and orthogonal experiments. The factors including pH, quantity of powdered activated carbon, decolouring temperature and adsorption time were investigated in terms of the decolouring rate and the reserved rate of peptide. The results showed that when the conditions were 40mL soybean meal protease hydrolysate, pH4. 0, quantity of powdered activated carbon 0. 6%, decolouring temperature 50℃ and adsorption time 50min, the decolorization effect was distinct but some peptide loss. The decolorizing rate was 86. 52%, the reserved rate of peptide was 75. 04%.