栗叶蛋白闪式提取工艺优化

Homogenate extraction process of leaf protein from leaves of Castanea mollissima

摘要: 探讨了闪式提取栗叶蛋白的工艺。运用Box-Behnken实验设计方法,在单因素实验的基础上,以栗叶蛋白得率为实验指标,研究料液比、pH、闪提温度以及闪提时间及其交互作用对栗叶蛋白得率的影响。实验结果表明,栗叶蛋白的最佳提取工艺参数为:料液比1:27（g/mL）,pH11,闪提温度50℃,闪提时间3.6min。在本实验中4个因素对栗叶蛋白提取得率作用的大小依次为:pH>闪提时间>闪提温度>料液比。在此条件下,栗叶蛋白提取得率达到11.89%。

Abstract: Homogenate extraction of leaf protein (LPC) from leaves of Castanea mollissima was studied. By the Box-Behnken experimental design method, the effects of the solid/liquid, pH, temperature and extraction time and their interactions on the LPC yield were studied on the base of the single-factor test. The test results showed that the optimum conditions of extraction for LPC were as following:the solid/liquid 1:27 (g/mL) , pH11, temperature 50℃, extraction time 3. 6min. The influence order of factors to protein extracting rate is pH>extraction time>temperature>solid/liquid. Under these conditions, the rate of LPC extraction reached 11. 89%