大孔树脂对樟子松树皮多酚的纯化工艺的研究

doi:10.13386/j.issn1002-0306.2013.11.024

大孔树脂对樟子松树皮多酚的纯化工艺的研究

Purification of polyphenols from pinus sylvestris L. var. mongolica barks by macroporous resin

摘要

摘要: 目的:优化樟子松树皮多酚纯化工艺,提高松多酚资源利用率。方法:以吸附率和解析率为衡量指标,确定纯化樟子松树皮多酚的最佳树脂;以多酚纯度和回收率为衡量指标,研究纯化过程中影响较大的因素,并且利用响应面优化樟子松树皮多酚的纯化工艺。结果:樟子松树皮多酚纯化的最佳工艺条件为:上样浓度3.0mg/mL,上样体积30mL,样液pH3.5,上样流速5BV/h,洗脱流速4.25BV/h,洗脱乙醇浓度53%。在此条件下,樟子松树皮多酚纯度可达67.91%,回收率为68.12%。结论:经D101大孔树脂纯化,樟子松树皮多酚纯度由37.25%升高至67.91%,说明D101大孔树脂对樟子松树皮多酚的纯化具有显著作用。

Abstract: Objective:Optimized the purification process of polyphenols from Pinus sylvestris L. bark and made the best use of its polyphenols resource. Method:The adsorption rate and desorption rate were detection indicators. Optimum resin for purification of polyphenols was determined. As the index to recovery and purity of polyphenols, influential factors were successively investigated, and the response surface design was used to optimize this process. Result:The optimum conditions of purification were sample solution concentration 3. 0mg/mL, 30mL sample solutionin in pH 3. 5, feeding speed 5BV/h, eluting speed 4. 25BV/h and the concentration of ethanol 53%. Purity of Pinus sylvestris L. bark polyphenols was 67. 91% and the recovery was 68. 12%. Conclusion:Purity of pinus sylvestris L. bark polyphenols was from 37. 25% to 67. 91% according to this purification condition, which indicated that D101 macroporous resin had distinct effect on purification of polyphenols from pinus sylvestris L. var. mongolica barks.