稀有人参皂苷compound K转化菌株的筛选及转化条件优化

Screening of phytopathogenic fungi by minor ginsenoside compound K production and optimization of biotransformation conditions

摘要: 从22种植物病原真菌中筛选出6种能够转化原人参二醇型皂苷为稀有人参皂苷compound K（C-K）的真菌,其中转化效率最高的1.91号真菌经形态学鉴定为卵形孢霉属（Oospora Wallr.）真菌。1.91号真菌生物转化人参皂苷Rb1和Rb2的途径分别为:Rb1→Rd→F2→C-K,Rb2→C-O→C-Y。1.91号真菌生物转化人参皂苷Rb1为C-K的最佳条件为:最佳底物添加时间是孢子预培养12h后,转化最适pH5.0～6.0,转化最适温度是45℃。本研究为稀有人参皂苷C-K的工业制备奠定了一定的基础。

Abstract: Twenty-two phytopathogenic fungi were screened with regard to their ability to produce minor ginsenoside compound K (C-K) . Six fungi were detected to be able to convert major protopanaxadiol type saponins to ginsenoside C-K in the all tested strains, and the No. 1. 91 fungal strain which showed the most efficient biotransformation was morphologically identified as Oospora Wallr. The biotransformation pathways of ginsenoside Rb1 and Rb2 by No. 1. 91 fungal strain were:Rb1→Rd→F2→C-K, Rb2→C-O→C-Y. The following optimum conditions for transforming C-K by No. 1. 91 fungal strain were determined:the time of substrate addition, 12h, pH5. 0 ～ 6. 0 and temperature 45℃. This study laid a certain foundation of the industrial preparation of minor ginsenoside C-K.