响应曲面法优化酪蛋白磷酸肽的制备

Optimization of the preparation of casein phosphopeptides by response surface methodology

摘要: 采用Plackett-Burman设计法和响应面分析法相结合,以CPP（酪蛋白磷酸肽）得率作为指标,进行碱性蛋白酶水解酪蛋白的条件优化。结果表明,底物浓度、温度和pH是影响CPP得率的主要因素。最佳水解条件为温度55℃、底物浓度16%、pH10.8、酶底比1.5%,水解时间4.5h,在此条件下,CPP得率可达33.2mg/mL。

Abstract: Plackett -burman and response surface analysis methods were applied to optimize conditions of alkaline protease hydrolyzing casein hydrolysis based on hydrolysis the CPP (Casein Phosphopeptides) content. The results indicated that CPP yield was mainly influenced by substrate concentration, temperature and pH. Optimum hydrolysis conditions as follows:temperature 55℃ , substrate concentration 16% , pH10.8, enzyme- substrate ratio 1.5%, time 4.5h. On this condition, the hydrolysis the CPP yield was 33.2mg/mL.